October 29, 2015

ZEBRAFISH

Zebrafish Embryo Model for Toxicology Evaluation

Integrated and conserved animal model which is represented dynamic animal model emerging the toxicological screening model test.

Why most scientists prefer Zebrafish Embryo Toxicity Testing as an alternative way to do toxicity testing?

In vitro model using cell line which is immortal modified is not natural and sensitive to certain condition such as the media required to grow the cell which contain fetal bovine serum. This introduces more bacteria and viruses. In addition, comparing to animal such as rodents, the cost is cheaper because it can be cultured in the minimal condition environment and needed asmaller space (eg: aquarium).

 
The development of zebrafish is robust and the larvae aretransparent. We can observe them under stereomicroscope and monitored them by hour-post-fertilization. For example, major organ precursors are observable within 36 hours and they will hatch after 72 hours and become larvae.
Zebrafish are highly similar to humans, there are roughly about 80% conservation of gene sequences. all these make the zebrafish a model organism for testing purposes.
By using zebrafish embryos, researchers can perform tests on whole vertebrate organisms at a relatively low cost and obtain result in shorter time.
Reducing animal testing is one of FDA’s stated goals in ISO 10993 and zebrafish is alternatively the crucial tool to bereplaced with rodent and other animals testing.
Since zebrafish can produce a thousand embryos at one time compare to rodent which is only a dozen, thus is able to reduce the time consumed and the amount of testing needed.