Keeping and raising of zebrafish stocks (wild-type short fin) in our facility is performed under permission of Institutional Animal Care and Use Committee (IACUC), Universiti Putra Malaysia. Adult zebrafish were maintained in an aquarium tank (1 fish/L) with continuous flowing water under temperature of approximately 25-28°C. Adult zebrafish were fed twice daily with brine shrimp salt, Artemia in the morning and dried flake food in the afternoon. The level of ammonia and pH of water were monitored frequently.
Images of normal zebrafish embryogenesis showing stages of development at different hours of post fertilization (hpf) captured using inverted microscope at 40X digital magnification.
a) Blastula period (4 hpf)
b) Segmentation period (24 hpf)
c) Pharyngula period (48 hpf)
d) Hatching period (72 hpf)
A – eye anlage; An – anus; Bc – blood cells; C – chorda; Ch – chorion; F – fin; G – gut; M – melanophores; O – ear bud; P – pericard; S – somites; Y – yolk sac.